8RBT
Emiliania huxleyi virus 201 (EhV-201) capsid proteins predicted by AlphaFold2 fitted into a cryo-EM density map of the EhV-201 virion capsid.
Replaces: 8PFMEM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Energy Filter | GIF Bioquantum |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SUBTOMOGRAM AVERAGING |
Resolution (Å) | 12 |
Software | emClarity,RELION,Warp,SerialEM,UCSF ChimeraX |
Symmetry | C5 |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |