8RB1
The crystal structure of DNA-bound human MutSbeta (MSH2_K675R/MSH3_K902R) in the canonical mismatch bound conformation with ADP bound in MSH2 and MSH3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-08 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.999812 |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.705, 91.681, 94.024 |
| Unit cell angles | 67.34, 87.16, 75.86 |
Refinement procedure
| Resolution | 86.660 - 2.085 |
| R-factor | 0.19893 |
| Rwork | 0.198 |
| R-free | 0.23539 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.362 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.660 | 2.121 |
| High resolution limit [Å] | 2.085 | 2.085 |
| Number of reflections | 119409 | 5968 |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 96.2 | |
| Redundancy | 2.2 | |
| CC(1/2) | 0.998 | 0.699 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 0.1 M MES pH 6.5 -7.5, 0.2 M Ammonium Acetate, 20 - 25 % PEG 3350 |
