8R9S
A soakable crystal form of human CDK7 in complex with AMP-PNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-X |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-08 |
Detector | DECTRIS EIGER R 1M |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.294, 145.584, 66.833 |
Unit cell angles | 90.00, 95.70, 90.00 |
Refinement procedure
Resolution | 35.120 - 2.780 |
R-factor | 0.24008 |
Rwork | 0.237 |
R-free | 0.29620 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.009 |
RMSD bond angle | 1.439 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | BUSTER |
Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.950 | 3.028 |
High resolution limit [Å] | 2.780 | 2.780 |
Rmeas | 0.300 | 0.632 |
Number of reflections | 12346 | 618 |
<I/σ(I)> | 3.54 | 1.3 |
Completeness [%] | 84.7 | |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% Glycerol .02M KNa tartrate .02M NH4 acetate .02M Na formate .02M Na oxamate .02M Na3 citrate 10% PEG 4000 .1M pH=7.5 MOPS/NaHEPES |