8R5C
Crystal structure of human TRIM7 PRYSPRY domain bound to (2-(1-oxoisoindolin-2-yl)-3-phenylpropanoyl)-L-glutamine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.716, 44.594, 59.657 |
| Unit cell angles | 90.00, 108.79, 90.00 |
Refinement procedure
| Resolution | 39.660 - 1.600 |
| R-factor | 0.1412 |
| Rwork | 0.139 |
| R-free | 0.17879 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.739 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.660 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.069 | 0.444 |
| Rmeas | 0.082 | 0.532 |
| Rpim | 0.044 | 0.290 |
| Total number of observations | 4524 | |
| Number of reflections | 27677 | 1367 |
| <I/σ(I)> | 9.3 | 1.8 |
| Completeness [%] | 99.9 | |
| Redundancy | 3.4 | 3.3 |
| CC(1/2) | 0.996 | 0.909 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1 M sodium tartrate, 100 mM citrate pH 5.9 |
