8R3Q
Transketolase from Plasmodium falciparum in complex with thiamin pyrophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-16 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.040, 114.340, 149.900 |
| Unit cell angles | 90.00, 90.11, 90.00 |
Refinement procedure
| Resolution | 46.810 - 1.880 |
| R-factor | 0.1454 |
| Rwork | 0.144 |
| R-free | 0.16790 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.900 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.810 | 1.900 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Number of reflections | 265236 | 8293 |
| <I/σ(I)> | 17.84 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.93 | |
| CC(1/2) | 0.998 | 0.847 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Li2SO4, 0.1 M Sodium citrate pH 5.6, 12 % (w/v |
