8R36
Crystal structure of the Gluk1 ligand-binding domain in complex with kainate and BPAM538 at 1.90 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX IV BEAMLINE BioMAX |
Synchrotron site | MAX IV |
Beamline | BioMAX |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 71.485, 71.485, 232.902 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.370 - 1.900 |
R-factor | 0.1659 |
Rwork | 0.164 |
R-free | 0.19930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e0x |
RMSD bond length | 0.009 |
RMSD bond angle | 0.808 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.400 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.087 | 0.457 |
Rmeas | 0.089 | 0.465 |
Rpim | 0.017 | 0.089 |
Total number of observations | 1291136 | 186128 |
Number of reflections | 48841 | 6995 |
<I/σ(I)> | 23.4 | 7.7 |
Completeness [%] | 100.0 | |
Redundancy | 26.4 | 26.6 |
CC(1/2) | 0.999 | 0.989 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 279 | 15.2% PEG4000, 0.3 M lithium sulfate, and 0.1 M sodium acetate pH 5.5 |