8R2G

Crystal structure of a BRCA2-DMC1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 41 2 2
Unit cell lengths	125.400, 125.400, 364.195
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	118.570 - 3.450
R-factor	0.2655
Rwork	0.264
R-free	0.30570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.480
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	182.098	3.692
High resolution limit [Å]	3.450	3.452
Rmeas	0.247	1.500
Rpim	0.072	0.561
Number of reflections	22688	1134
<I/σ(I)>	8	1.5
Completeness [%]	57.7	16.1
Redundancy	12.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	50 mM HEPES-NaOH pH 7.4, 50 mM MgCl2, 500 mM NaCl, 8 % PEG 3350, 20 % glycerol