8R2G
Crystal structure of a BRCA2-DMC1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 125.400, 125.400, 364.195 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 118.570 - 3.450 |
R-factor | 0.2655 |
Rwork | 0.264 |
R-free | 0.30570 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.480 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 182.098 | 3.692 |
High resolution limit [Å] | 3.450 | 3.452 |
Rmeas | 0.247 | 1.500 |
Rpim | 0.072 | 0.561 |
Number of reflections | 22688 | 1134 |
<I/σ(I)> | 8 | 1.5 |
Completeness [%] | 57.7 | 16.1 |
Redundancy | 12.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 50 mM HEPES-NaOH pH 7.4, 50 mM MgCl2, 500 mM NaCl, 8 % PEG 3350, 20 % glycerol |