8R0Z

14-3-3 sigma in complex with TAZ peptide and stabilizing fragment TCF199

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97626
Spacegroup name	C 2 2 21
Unit cell lengths	81.474, 111.984, 62.754
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.880 - 1.200
R-factor	0.169
Rwork	0.169
R-free	0.17560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.911
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.880	1.220
High resolution limit [Å]	1.200	1.200
Rmerge	0.075	0.529
Rmeas	0.081	0.575
Number of reflections	89634	8853
<I/σ(I)>	17.4
Completeness [%]	100.0
Redundancy	12.8
CC(1/2)	0.997	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	0.095M HEPES pH 7.5, 26%PEG 400, 0.19M CaCL, 5%Glycerol