8QZ1

Crystal structure of human two pore domain potassium ion channel TREK-2 (K2P10.1) in complex with a nanobody (Nb58)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9686
Spacegroup name	H 3
Unit cell lengths	233.663, 233.663, 67.388
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.840 - 3.588
R-factor	0.2579
Rwork	0.256
R-free	0.29840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.710
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.158	3.691
High resolution limit [Å]	3.591	3.591
Rmeas	0.116	1.339
Rpim	0.051	0.649
Number of reflections	14181	708
<I/σ(I)>	8.6	1.3
Completeness [%]	88.6	55.7
Redundancy	5.2	4.1
CC(1/2)	0.996	0.511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10	277	0.1 M glycine pH 10.0, 0.2 M NaCl, 31% v/v polyethylene glycol 1000