8QZ1
Crystal structure of human two pore domain potassium ion channel TREK-2 (K2P10.1) in complex with a nanobody (Nb58)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | H 3 |
| Unit cell lengths | 233.663, 233.663, 67.388 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.840 - 3.588 |
| R-factor | 0.2579 |
| Rwork | 0.256 |
| R-free | 0.29840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.710 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.158 | 3.691 |
| High resolution limit [Å] | 3.591 | 3.591 |
| Rmeas | 0.116 | 1.339 |
| Rpim | 0.051 | 0.649 |
| Number of reflections | 14181 | 708 |
| <I/σ(I)> | 8.6 | 1.3 |
| Completeness [%] | 88.6 | 55.7 |
| Redundancy | 5.2 | 4.1 |
| CC(1/2) | 0.996 | 0.511 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10 | 277 | 0.1 M glycine pH 10.0, 0.2 M NaCl, 31% v/v polyethylene glycol 1000 |
