8QYT
Human Pyridoxine-5'-phosphate oxidase in complex with PLP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 82.480, 82.480, 59.190 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.600 - 1.690 |
| R-factor | 0.17505 |
| Rwork | 0.173 |
| R-free | 0.21563 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.664 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.600 | 1.720 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 1.443 | |
| Rmeas | 0.050 | |
| Number of reflections | 26324 | 1316 |
| <I/σ(I)> | 30.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 18.4 | 18.8 |
| CC(1/2) | 1.000 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 12% vv PEG 6000, 0.1M Sodium Citrate, 0.1M Lithium Sulfate |
