8QYA

J22.9-FNY, fully humanized, CDR optimized Fab Fragment based on chimeric J22.9-xi IgG against BCMA; with VH CDR2 glycosylation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	C 1 2 1
Unit cell lengths	137.266, 55.371, 69.486
Unit cell angles	90.00, 108.01, 90.00

Refinement procedure

Resolution	21.760 - 2.720
R-factor	0.2467
Rwork	0.244
R-free	0.29680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.450
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.760	2.817
High resolution limit [Å]	2.720	2.720
Number of reflections	13486	1323
<I/σ(I)>	9.98
Completeness [%]	99.4
Redundancy	3.6
CC(1/2)	0.993	0.747

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG 3350 CuCl2 BisTris