8QYA
J22.9-FNY, fully humanized, CDR optimized Fab Fragment based on chimeric J22.9-xi IgG against BCMA; with VH CDR2 glycosylation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 137.266, 55.371, 69.486 |
Unit cell angles | 90.00, 108.01, 90.00 |
Refinement procedure
Resolution | 21.760 - 2.720 |
R-factor | 0.2467 |
Rwork | 0.244 |
R-free | 0.29680 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.450 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 21.760 | 2.817 |
High resolution limit [Å] | 2.720 | 2.720 |
Number of reflections | 13486 | 1323 |
<I/σ(I)> | 9.98 | |
Completeness [%] | 99.4 | |
Redundancy | 3.6 | |
CC(1/2) | 0.993 | 0.747 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 3350 CuCl2 BisTris |