8QY9

J22.9-H, fully humanized Fab Fragment based on chimeric J22.9-xi IgG against BCMA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	C 1 2 1
Unit cell lengths	137.360, 55.180, 81.160
Unit cell angles	90.00, 121.02, 90.00

Refinement procedure

Resolution	34.780 - 3.100
R-factor	0.256
Rwork	0.254
R-free	0.30110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.445
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21.1-5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.780	3.211
High resolution limit [Å]	3.100	3.100
Number of reflections	9584	942
<I/σ(I)>	6.54
Completeness [%]	99.2	99.37
Redundancy	4.14
CC(1/2)	0.985	0.611

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293.15	14% PEG 3350 100 mM BisTris 7.5 mM Cu(II)