8QWO
Structure of p53 cancer mutant Y234C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-03-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.059, 70.987, 105.127 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.960 - 1.380 |
R-factor | 0.152920350342 |
Rwork | 0.152 |
R-free | 0.17796 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.005 |
RMSD bond angle | 0.774 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.960 | 1.400 |
High resolution limit [Å] | 1.380 | 1.380 |
Rmerge | 0.053 | 0.938 |
Number of reflections | 100451 | 4906 |
<I/σ(I)> | 17.1 | 2.3 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 6.6 | 6.8 |
CC(1/2) | 0.999 | 0.874 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Protein solution: 5.5-6.0 mg/ml in 25 mM phosphate (pH 7.5), 150 mM NaCl, 0.5 mM TCEP Reservoir buffer: 19% PEG 4000 (w/v), 0.1 M HEPES (pH 7.0), 5 mM DTT. |