8QUW

Hexameric HIV-1 CA in complex with DDD01044153

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-02
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 6
Unit cell lengths	90.472, 90.472, 56.688
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.340 - 2.020
R-factor	0.19133
Rwork	0.189
R-free	0.23048
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.770
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.340	2.130
High resolution limit [Å]	2.020	2.020
Rmerge	0.120	0.470
Number of reflections	17180	2246
<I/σ(I)>	11	3
Completeness [%]	98.5	89.7
Redundancy	5.8	4.3
CC(1/2)	0.990	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	0.1M Tris buffer, pH 8.0 to 9.0, 10-15% PEG550MME, 0.15M KSCN