8QRW

ASCT2 protomer in lipid nanodiscs under low Na+ concentration in the intermediate outward-facing state (iOFS-up)

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	FEI TALOS ARCTICA
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	200
Cs (mm)	2.7
Energy Filter	GIF Bioquantum
Detector	GATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	3
Number of Particle	3256089
Software	crYOLO,cryoSPARC,RELION,PHENIX,SerialEM,CTFFIND,Coot,ISOLDE
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION