8QQL
Cryo-EM structure of the human inward-rectifier potassium 2.1 channel (Kir2.1) - R312H mutant
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | TFS Selectris X |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 6 |
| Number of Particle | 2787753 |
| Software | cryoSPARC,PHENIX,EPU,NAMD |
| Symmetry | C4 |
| CTF correction | NONE |
