8QQE
Crystal structure of the complex between DMC1 and the PhePP domain of BRCA2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-02 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.965459 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 234.383, 234.383, 234.383 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.840 - 3.461 |
| R-factor | 0.2491 |
| Rwork | 0.248 |
| R-free | 0.26090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.800 |
| Data reduction software | XDS (20220820) |
| Data scaling software | STARANISO |
| Phasing software | MOLREP (11.9.02) |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.840 | 3.550 |
| High resolution limit [Å] | 3.460 | 3.460 |
| Rmerge | 0.228 | 6.345 |
| Rmeas | 0.229 | 6.387 |
| Rpim | 0.026 | 0.728 |
| Number of reflections | 14667 | 1058 |
| <I/σ(I)> | 22.9 | 1.1 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 76.3 | 76.3 |
| CC(1/2) | 1.000 | 0.541 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | 0.1M MES at pH 6, 2.4 M sodium formate |
