8QP7

Crystal structure of Hepatitis C Virus E2 glycoprotein epitopeI 411-424 scaffold design 4CIL_04

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.999995
Spacegroup name	C 1 2 1
Unit cell lengths	60.290, 31.670, 135.750
Unit cell angles	90.00, 94.31, 90.00

Refinement procedure

Resolution	30.060 - 2.050
R-factor	0.2168
Rwork	0.214
R-free	0.26170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.468
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.18_3861)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.060	2.123
High resolution limit [Å]	2.050	2.050
Number of reflections	16198	1652
<I/σ(I)>	9.85	2.54
Completeness [%]	97.2	95.02
Redundancy	4.5
CC(1/2)	0.980	0.323

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	25% (w/v) PEG 1500, 100 mM MMT buffer pH 9.0