8QKT
Structure of a nucleosome composed of a palindromic 167-base pair blunt-ended DNA fragment
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-10 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 108.437, 103.722, 185.405 |
| Unit cell angles | 90.00, 93.59, 90.00 |
Refinement procedure
| Resolution | 48.655 - 3.261 |
| Rwork | 0.269 |
| R-free | 0.32100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.232 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.660 | 3.440 |
| High resolution limit [Å] | 3.260 | 3.260 |
| Number of reflections | 61655 | 7836 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 96.0 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.997 | 0.541 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | MnCl2-containing buffers |
