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8QKT

Structure of a nucleosome composed of a palindromic 167-base pair blunt-ended DNA fragment

This is a non-PDB format compatible entry.
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06DA
Synchrotron siteSLS
BeamlineX06DA
Temperature [K]100
Detector technologyPIXEL
Collection date2014-10-10
DetectorDECTRIS PILATUS 2M-F
Wavelength(s)0.9
Spacegroup nameP 1 21 1
Unit cell lengths108.437, 103.722, 185.405
Unit cell angles90.00, 93.59, 90.00
Refinement procedure
Resolution48.655 - 3.261
Rwork0.269
R-free0.32100
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.009
RMSD bond angle1.232
Data reduction softwareXDS
Data scaling softwareSCALA
Phasing softwareMOLREP
Refinement softwareREFMAC (5.8.0258)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]48.6603.440
High resolution limit [Å]3.2603.260
Number of reflections616557836
<I/σ(I)>8.9
Completeness [%]96.0
Redundancy3.2
CC(1/2)0.9970.541
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP291MnCl2-containing buffers

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