8QJT
BRM (SMARCA2) Bromodomain in complex with ligand 10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-12-13 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 31 |
| Unit cell lengths | 63.750, 63.750, 88.510 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 55.210 - 2.568 |
| R-factor | 0.2054 |
| Rwork | 0.203 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.940 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.210 | 2.610 |
| High resolution limit [Å] | 2.570 | 2.570 |
| Rmerge | 0.144 | 0.932 |
| Number of reflections | 12685 | 646 |
| <I/σ(I)> | 9.5 | 2.1 |
| Completeness [%] | 98.9 | 100 |
| Redundancy | 5.4 | 5.4 |
| CC(1/2) | 0.996 | 0.829 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 20% PEG 6K, 6% ethylene glycol, 10 mM ZnCl2 |
