8QHN
Streptococcus pyogenes GapN in complex with NADPH and erythrose-4-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-09 |
| Detector | DECTRIS PILATUS 12M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 |
| Unit cell lengths | 97.521, 98.476, 102.981 |
| Unit cell angles | 77.77, 76.08, 67.79 |
Refinement procedure
| Resolution | 41.150 - 1.990 |
| R-factor | 0.1684 |
| Rwork | 0.166 |
| R-free | 0.21080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.544 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.404 | 2.112 |
| High resolution limit [Å] | 1.989 | 1.989 |
| Rmerge | 0.093 | 0.564 |
| Rmeas | 0.108 | 0.658 |
| Rpim | 0.055 | 0.336 |
| Number of reflections | 186084 | 9305 |
| <I/σ(I)> | 7.403 | 1.545 |
| Completeness [%] | 79.3 | 24.01 |
| Redundancy | 3.72 | 3.73 |
| CC(1/2) | 0.998 | 0.757 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 295 | Precipitant: 0.01 M PBS buffer 0.16-0.185 M (NH4)3-citrate 18-21% (w/v) PEG 3350 1 mM E4P Protein concentration: 8 mg/ml in PBS buffer |
