8QHM
DNA mimic Foldamer with sticky ends
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-18 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 74.146, 74.146, 83.693 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.970 - 3.000 |
| R-factor | 0.349 |
| Rwork | 0.347 |
| R-free | 0.38120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.029 |
| RMSD bond angle | 5.628 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.970 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 4874 | 395 |
| <I/σ(I)> | 15.98 | 5.02 |
| Completeness [%] | 94.5 | 81.28 |
| Redundancy | 4.3 | 4.1 |
| CC(1/2) | 0.998 | 0.984 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293.15 | 20% PEG 6K, 0.1M HEPES ph 7.0, 200mM Nacl |
