8QH1
Crystal structure of the SARS-CoV-2 RBD from the Omicron BA4 variant with the antibody Cv2.3194
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.919 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 57.793, 85.348, 193.987 |
| Unit cell angles | 90.00, 97.48, 90.00 |
Refinement procedure
| Resolution | 39.010 - 2.650 |
| R-factor | 0.1994 |
| Rwork | 0.198 |
| R-free | 0.23230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.529 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.010 | 2.780 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.094 | 1.489 |
| Rpim | 0.060 | 0.952 |
| Number of reflections | 27213 | 3610 |
| <I/σ(I)> | 6.7 | |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% PEG 3350, 0.2 M sodium malonate |
