8QFZ
TSLP-Bicycle complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-16 |
Detector | DECTRIS PILATUS 12M |
Wavelength(s) | 0.976 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.920, 52.349, 65.829 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.970 - 1.650 |
R-factor | 0.18747 |
Rwork | 0.186 |
R-free | 0.20975 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 2.102 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135 STEINER) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.970 | 1.570 |
High resolution limit [Å] | 1.520 | 1.520 |
Rmerge | 0.090 | 0.610 |
Rmeas | 0.099 | 0.754 |
Rpim | 0.040 | 0.429 |
Total number of observations | 129247 | 4434 |
Number of reflections | 23603 | 1652 |
<I/σ(I)> | 10.1 | 1.7 |
Completeness [%] | 93.9 | |
Redundancy | 5.5 | 2.7 |
CC(1/2) | 0.994 | 0.534 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 30% PEG MME 500/PEG 20000, 0.1 M Carboxylic acids mix and 0.1 M Buffer system 3 pH 8.5 |