8Q9Z
Cryo-EM structure of Cx26 gap junction K125E mutant in bicarbonate buffer (classification on hemichannel)
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.4 |
| Number of Particle | 1104398 |
| Software | RELION,CTFFIND,Coot,PHENIX |
| Symmetry | C6 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
