8Q9W
Crystal structure of HsRNMT complexed with sinefungin and GMP-PnP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.463, 84.086, 85.344 |
| Unit cell angles | 90.00, 99.67, 90.00 |
Refinement procedure
| Resolution | 42.040 - 2.500 |
| R-factor | 0.1769 |
| Rwork | 0.174 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.667 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.040 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.140 | 0.890 |
| Number of reflections | 19534 | 2178 |
| <I/σ(I)> | 8.1 | 3.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 4.7 | 4.7 |
| CC(1/2) | 0.980 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 0.1 M MES pH 6.3-6.9, 50 mM Na2SO4, 18-25 % PEG 6000 |
