8Q9Q

Crystal Structure of the MADS-box/MEF2 Domain of MEF2D bound to dsDNA and HDAC7 deacetylase binding motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-09-11
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	56.883, 59.361, 76.502
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.940 - 2.110
R-factor	0.26635
Rwork	0.265
R-free	0.28489
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.705
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.500	2.170
High resolution limit [Å]	2.110	2.110
Rmerge	0.089	0.732
Number of reflections	14910	1222
<I/σ(I)>	11
Completeness [%]	96.0	98.9
Redundancy	8.8	2.5
CC(1/2)	0.999	0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM sodium acetate trihydrate, 100 mM Bis-Tris propane pH 6.5 and 20% w/v polyethylene glycol 3350 (PEG 3350)