8Q9P

Crystal Structure of the MADS-box/MEF2 Domain of MEF2D bound to dsDNA and HDAC5 deacetylase binding motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-13
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.873
Spacegroup name	P 21 21 21
Unit cell lengths	54.243, 56.748, 76.689
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.620 - 2.200
R-factor	0.20384
Rwork	0.201
R-free	0.26208
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.644
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.620	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.044	0.306
Number of reflections	12557	1071
<I/σ(I)>	9.8
Completeness [%]	100.0
Redundancy	1.9
CC(1/2)	0.991	0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM sodium formate, 100 mM Bis-Tris propane pH 6.5 and 20% w/v polyethylene glycol 3350 (PEG 3350)