8Q7K

IRGQ LIR2 peptide in complex with LC3B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-08-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9999
Spacegroup name	P 43 21 2
Unit cell lengths	68.423, 68.423, 118.071
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.190 - 1.600
R-factor	0.1927
Rwork	0.192
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.167
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.380	1.657
High resolution limit [Å]	1.600	1.600
Number of reflections	37749	7386
<I/σ(I)>	19.66
Completeness [%]	99.9
Redundancy	2
CC(1/2)	1.000	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	1 M lithium chloride, 0.1 M citrate, 20% PEG 6000