8Q5D

PfRH5 bound to monoclonal antibody MAD10-466

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-26
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.95374
Spacegroup name	P 1 21 1
Unit cell lengths	82.464, 140.414, 83.616
Unit cell angles	90.00, 112.74, 90.00

Refinement procedure

Resolution	67.590 - 3.200
R-factor	0.2742
Rwork	0.272
R-free	0.31670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.920
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	BUSTER (2.10.4 (26-JUL-2023))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	67.590	3.260
High resolution limit [Å]	3.200	3.200
Number of reflections	28799	1404
<I/σ(I)>	2.3
Completeness [%]	99.4
Redundancy	3.6
CC(1/2)	0.952	0.345

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Buffer System 3 pH 8.5, 0.1 M Amino Acids, 30% v/v Precipitant Mix 1