8Q44
Crystal structure of cA4-bound Can2 (E364R) in complex with oligo-T DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-05-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.714, 78.357, 94.661 |
| Unit cell angles | 90.00, 95.35, 90.00 |
Refinement procedure
| Resolution | 57.460 - 2.300 |
| R-factor | 0.1996 |
| Rwork | 0.198 |
| R-free | 0.22640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS (20220110) |
| Data scaling software | autoPROC (20230726) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.460 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.079 | 0.771 |
| Rmeas | 0.085 | 0.834 |
| Rpim | 0.033 | 0.314 |
| Number of reflections | 37158 | 3660 |
| <I/σ(I)> | 13.22 | 2.46 |
| Completeness [%] | 99.0 | 98.62 |
| Redundancy | 6.8 | 6.8 |
| CC(1/2) | 0.998 | 0.854 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 50 mM Tris pH 8, 17.1% (v/v) PEG 550 MME, 4.3% (w/v) PEG 2000 |
