8Q40
Crystal structure of cA4 activated Can2 in complex with a cleaved DNA substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-11-09 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.696, 79.062, 94.579 |
| Unit cell angles | 90.00, 95.36, 90.00 |
Refinement procedure
| Resolution | 47.120 - 2.210 |
| R-factor | 0.1983 |
| Rwork | 0.196 |
| R-free | 0.23590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.629 |
| Data reduction software | XDS (20220110) |
| Data scaling software | autoPROC (20230726) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.120 | 2.289 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.068 | 0.759 |
| Rmeas | 0.074 | 0.827 |
| Rpim | 0.029 | 0.323 |
| Number of reflections | 37882 | 2076 |
| <I/σ(I)> | 16.52 | 2.24 |
| Completeness [%] | 88.7 | 48.97 |
| Redundancy | 6.4 | 6.2 |
| CC(1/2) | 0.999 | 0.867 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 50 mM Tris pH 8, 17.1% (v/v) PEG 550 MME, 4.3% (w/v) PEG 2000 |
