8Q3X

Structure of Nucleosome Core with a Bound Metallopeptide Conjugate (Kaposi Sarcoma Associated Herpesvirus LANA Peptide-Au[I] Compound)

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	98
Detector technology	PIXEL
Collection date	2018-09-03
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.04
Spacegroup name	P 21 21 21
Unit cell lengths	108.105, 109.609, 183.767
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.930 - 2.301
Rwork	0.224
R-free	0.26230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.474
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.930	2.430
High resolution limit [Å]	2.300	2.300
Number of reflections	97275	13946
<I/σ(I)>	27.3
Completeness [%]	99.9
Redundancy	12.2
CC(1/2)	1.000	0.651

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Buffers containing MnCl2, KCl and K-cacodylate [pH 6.0]