8Q2G

X-ray structure of LysECD7 endolysin against Gram-negative bacteria

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-20
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9184
Spacegroup name	C 2 2 2
Unit cell lengths	75.568, 82.334, 64.604
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.174 - 2.490
Rwork	0.268
R-free	0.35800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	1.175
Data reduction software	XDS
Data scaling software	XDS
Phasing software	Arcimboldo
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.174	2.560
High resolution limit [Å]	2.490	2.490
Number of reflections	10864	3326
<I/σ(I)>	13.04
Completeness [%]	97.4	93.83
Redundancy	6
CC(1/2)	0.990	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.2 M Ammonium sulfate, 20% w/v Polyethylene glycol 3,350 pH 6.0