8Q1H
LSD1 Y391K-CoREST bound to Histone H3 N-terminal tail
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.965459 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 118.061, 179.354, 233.055 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.860 - 2.900 |
R-factor | 0.2334 |
Rwork | 0.233 |
R-free | 0.25440 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.181 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.19.2_4158)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.860 | 2.980 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.133 | 1.786 |
Rmeas | 0.152 | 2.043 |
Rpim | 0.072 | 0.972 |
Number of reflections | 54837 | 4461 |
<I/σ(I)> | 8 | 1.1 |
Completeness [%] | 99.6 | 99.7 |
Redundancy | 4.4 | 4.4 |
CC(1/2) | 0.994 | 0.383 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | Sodium Tartrate 1.2 M, ADA 0.1M |