8Q1H

LSD1 Y391K-CoREST bound to Histone H3 N-terminal tail

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.965459
Spacegroup name	I 2 2 2
Unit cell lengths	118.061, 179.354, 233.055
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.860 - 2.900
R-factor	0.2334
Rwork	0.233
R-free	0.25440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.181
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.860	2.980
High resolution limit [Å]	2.900	2.900
Rmerge	0.133	1.786
Rmeas	0.152	2.043
Rpim	0.072	0.972
Number of reflections	54837	4461
<I/σ(I)>	8	1.1
Completeness [%]	99.6	99.7
Redundancy	4.4	4.4
CC(1/2)	0.994	0.383

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	Sodium Tartrate 1.2 M, ADA 0.1M