8PXV

Targeting extended blood antigens by Akkermansia muciniphila enzymes unveils a missing link for generating universal donor blood

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	96.260, 170.420, 171.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.320 - 2.500
R-factor	0.1799
Rwork	0.178
R-free	0.21220
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.669
Data reduction software	xia2
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.320	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.146
Rmeas	0.163
Rpim	0.069	0.658
Number of reflections	93815	9509
<I/σ(I)>	10.36	1.34
Completeness [%]	95.3
Redundancy	4.8
CC(1/2)	0.995	0.457

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	11% PEG8000, 0.5 M NaCl, 0.1M NaCacodylate