8PWX

PfRH5 bound to monoclonal antibody R5.008

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-08-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9999
Spacegroup name	P 31 2 1
Unit cell lengths	114.990, 114.990, 147.494
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.790 - 3.200
R-factor	0.2429
Rwork	0.240
R-free	0.29350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.493
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.490	3.420
High resolution limit [Å]	3.200	3.200
Number of reflections	19138	3408
<I/σ(I)>	5.2
Completeness [%]	100.0
Redundancy	5.2
CC(1/2)	0.986	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.12 M Ethylene Glycol Mix (di-, tri-, tetra- and pentaethylene glycols) 0.1 M Buffer System 3 pH 8.5 (BICINE, Trizma base) 30% v/v Precipitant Mix 3 (glycerol, PEG 4000)