8PWC

Crystal structure of MDM2 with Brigimadlin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 32
Unit cell lengths	83.207, 83.207, 42.056
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.600 - 1.461
R-factor	0.2007
Rwork	0.199
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.040
Data reduction software	autoPROC
Data scaling software	autoPROC ((Version 1.1.7))
Phasing software	BUSTER
Refinement software	BUSTER (2.11.8 (8-JUN-2022))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.600	1.553
High resolution limit [Å]	1.461	1.461
Rpim	0.029
Number of reflections	45565	2282
<I/σ(I)>	11.9
Completeness [%]	80.8
Redundancy	9.4
CC(1/2)	0.997	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	22% PEG 3350, 0.1M sodium sulphate, 10 mM manganes chloride