8PW9

Crystal structure of the human METTL3-METTL14 in complex with a bisubstrate analogue (BA1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-12
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	63.940, 63.940, 225.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.690 - 2.300
R-factor	0.1956
Rwork	0.194
R-free	0.23540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.997
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.700	2.440
High resolution limit [Å]	2.300	2.300
Number of reflections	24705	3897
<I/σ(I)>	17.35
Completeness [%]	99.9
Redundancy	9.6
CC(1/2)	0.999	0.687

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	20% PEG 3350, 0.4M Magnesium acetate