8PNL
Outward-open conformation of a Major Facilitator Superfamily (MFS) transporter MHAS2168, a homologue of Rv1410 from M. tuberculosis, in complex with an alpaca nanobody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-08-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.999 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 57.780, 160.700, 82.960 |
Unit cell angles | 90.00, 109.01, 90.00 |
Refinement procedure
Resolution | 44.700 - 2.700 |
R-factor | 0.2474 |
Rwork | 0.245 |
R-free | 0.29150 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.729 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.700 | 50.000 | 2.770 |
High resolution limit [Å] | 2.700 | 12.070 | 2.700 |
Rmerge | 0.095 | 0.026 | 1.638 |
Rmeas | 0.107 | 0.029 | 1.838 |
Number of reflections | 39032 | 440 | 2911 |
<I/σ(I)> | 8.77 | ||
Completeness [%] | 99.3 | ||
Redundancy | 4.65 | ||
CC(1/2) | 0.999 | 0.999 | 0.347 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5.7 | 293.15 | 380 mM NaH2PO4, 0.1 M sodium citrate (pH 5.7), 28% (v/v) PEG400, 2.4% (v/v) 1,4-butanediole |