8PMX
rat HEV P domain in complex with glycan-sensitive nAb p60.12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.99999 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 112.856, 112.856, 184.439 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.680 - 3.922 |
| R-factor | 0.3038 |
| Rwork | 0.300 |
| R-free | 0.36150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.860 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.680 | 4.130 |
| High resolution limit [Å] | 3.890 | 3.890 |
| Rmeas | 0.322 | |
| Number of reflections | 20832 | 3268 |
| <I/σ(I)> | 7.68 | |
| Completeness [%] | 99.6 | |
| Redundancy | 14.23 | |
| CC(1/2) | 0.998 | 0.401 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% w/v PEG 3350, 0.2M di-Ammonium hydrogen phospahate |
