8PMK
Cryo-EM structure of active Phthaloyl-CoA decarboxylase (Pcd) complex with prFMN bound
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.43 |
| Number of Particle | 2206933 |
| Software | crYOLO,RELION,PHENIX,EPU,Gctf,Coot |
| Symmetry | D3 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
