8PK5

INTS13-INTS14 complex with ZNF609

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-24
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000
Spacegroup name	P 21 21 2
Unit cell lengths	189.065, 115.629, 147.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.940 - 2.500
R-factor	0.1913
Rwork	0.190
R-free	0.21430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6sn1
RMSD bond length	0.008
RMSD bond angle	1.000
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.940	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	112597	11101
<I/σ(I)>	16.57
Completeness [%]	99.9
Redundancy	13.6
CC(1/2)	0.999	0.145

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Hepes-NaOH pH 6.5 0.9 M Na-Malonate 0.25 % (v/v) Jeffamine ED-2003