8PIW

Crystal structure of Hen Egg White Lysozyme co-crystallized with 10 mM TbXo4-NMet2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.6480
Spacegroup name	P 43 21 2
Unit cell lengths	77.521, 77.521, 37.793
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.760 - 1.720
R-factor	0.1731
Rwork	0.172
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.999
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.760	1.820
High resolution limit [Å]	1.720	1.720
Rmerge	0.083	0.617
Rpim	0.024	0.138
Number of reflections	12670	1776
<I/σ(I)>	0.242	0.062
Completeness [%]	99.9	99.4
Redundancy	24.2	23.5
CC(1/2)	0.998	0.965

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	950 mM sodium chloride, 100mM sodium acetate pH=4.6. Drops were set by mixing one volume of protein solution at 20mg/ml with one volume of 10mM TbXo4 and with one volume of the well solution.