8PII
VHH Z70 mutant 3 in interaction with PHF6 Tau peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-06-16 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 135.435, 135.435, 35.425 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.330 - 2.350 |
Rwork | 0.200 |
R-free | 0.26320 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.390 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.330 | 44.330 | 2.430 |
High resolution limit [Å] | 2.350 | 9.100 | 2.350 |
Rmerge | 0.203 | 0.058 | 2.227 |
Rmeas | 0.208 | 0.060 | 2.281 |
Rpim | 0.045 | 0.013 | 0.490 |
Number of reflections | 8432 | 187 | 789 |
<I/σ(I)> | 18.8 | ||
Completeness [%] | 100.0 | ||
Redundancy | 38.7 | 29.5 | 40.2 |
CC(1/2) | 0.999 | 0.999 | 0.947 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.5M ammonium sulfate, 4.5% isopropanol, 15% glycerol |