8PI4
Crystal structure of human insulin desB30 precursor with an Alanine-Methionine-Lysine C-peptide in dimer (T2) conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-16 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 21 3 |
| Unit cell lengths | 77.400, 77.400, 77.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.700 - 1.250 |
| R-factor | 0.1733 |
| Rwork | 0.173 |
| R-free | 0.17550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.063 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.700 | 1.290 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.052 | 1.391 |
| Rmeas | 0.053 | 1.428 |
| Rpim | 0.012 | 0.320 |
| Number of reflections | 21467 | 2099 |
| <I/σ(I)> | 25.55 | 1.85 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.7 | 19.8 |
| CC(1/2) | 1.000 | 0.770 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2 M sodium acetate trihydrate, 0.1 M Tris, pH 8.5, 30 % (w/v) PEG 4000 |
