8PHV
Structure of Human selenomethionylated Cdc123 bound to domain 3 of eIF2 gamma
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-10 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.259, 125.340, 74.081 |
| Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
| Resolution | 60.260 - 1.970 |
| R-factor | 0.1892 |
| Rwork | 0.186 |
| R-free | 0.22160 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.736 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.260 | 2.156 |
| High resolution limit [Å] | 1.970 | 1.974 |
| Rmeas | 0.078 | 1.098 |
| Number of reflections | 58305 | 2915 |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 94.1 | 63.7 |
| Redundancy | 7 | 6.9 |
| CC(1/2) | 0.997 | 0.650 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% PEG3350, 8% tacsimate |
