8PHI

Crystal structure of prefusion-stabilized RSV F Variant DS-Cav1 in complex with Lonafarnib

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91983
Spacegroup name	H 3
Unit cell lengths	88.640, 88.640, 194.980
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.210 - 2.289
R-factor	0.1998
Rwork	0.198
R-free	0.22850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	XSCALE (Jan 10, 2022)
Phasing software	PHASER (1.20.1-4487)
Refinement software	BUSTER (2.10.4 (17-FEB-2023))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.350
High resolution limit [Å]	2.289	2.289
Rmeas	0.243	1.755
Number of reflections	51465	3770
<I/σ(I)>	10.54	1.66
Completeness [%]	99.9	99
Redundancy	26.1
CC(1/2)	0.997	0.882

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291.15	PEG 6000-20%, CaCl2-400mM, & TrisHCl pH-8 100mM