8PHI
Crystal structure of prefusion-stabilized RSV F Variant DS-Cav1 in complex with Lonafarnib
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-04-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91983 |
Spacegroup name | H 3 |
Unit cell lengths | 88.640, 88.640, 194.980 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.210 - 2.289 |
R-factor | 0.1998 |
Rwork | 0.198 |
R-free | 0.22850 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.980 |
Data reduction software | XDS |
Data scaling software | XSCALE (Jan 10, 2022) |
Phasing software | PHASER (1.20.1-4487) |
Refinement software | BUSTER (2.10.4 (17-FEB-2023)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.350 |
High resolution limit [Å] | 2.289 | 2.289 |
Rmeas | 0.243 | 1.755 |
Number of reflections | 51465 | 3770 |
<I/σ(I)> | 10.54 | 1.66 |
Completeness [%] | 99.9 | 99 |
Redundancy | 26.1 | |
CC(1/2) | 0.997 | 0.882 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291.15 | PEG 6000-20%, CaCl2-400mM, & TrisHCl pH-8 100mM |