8PFL
Crystal structure of WRN helicase domain in complex with 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-16 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.231, 87.484, 133.878 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.260 - 1.800 |
| R-factor | 0.1983 |
| Rwork | 0.197 |
| R-free | 0.22550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.910 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.700 | 1.830 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.066 | 1.096 |
| Rmeas | 0.072 | 1.191 |
| Number of reflections | 44341 | 2217 |
| <I/σ(I)> | 15 | 1.6 |
| Completeness [%] | 95.8 | |
| Redundancy | 6.7 | |
| CC(1/2) | 0.999 | 0.643 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% Jeffamine ED-2001 0.1 M Hepes pH 7 |
