8PF0
Diubiquitin-derived artificial binding protein (Affilin) variant Af1 specific to oncofetal fibronectin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-05-28 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9184 |
Spacegroup name | P 41 |
Unit cell lengths | 62.767, 62.767, 67.841 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.050 - 2.200 |
R-factor | 0.195 |
Rwork | 0.193 |
R-free | 0.22610 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.089 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHARP |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.300 |
High resolution limit [Å] | 2.200 | 10.000 | 2.200 |
Rmerge | 0.031 | 0.033 | 0.387 |
Rmeas | 0.036 | 0.038 | 0.451 |
Number of reflections | 26176 | 240 | 3189 |
<I/σ(I)> | 22.88 | ||
Completeness [%] | 99.5 | ||
Redundancy | 3.8 | ||
CC(1/2) | 0.999 | 0.997 | 0.910 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 288 | 100 mM Imidazol/MES, 60 mM calcium/magnesium chloride, 30% PEG 8000/ethylene glycol (w/v), 10 mM copper(II)chloride dehydrate |